This performance approaches the critical NT-proBNP concentration threshold often used by physicians for risk stratification purposes: ~0.116 nM. Finally, and most important, the assay is able to detect NT-proBNP at concentrations between 0.58 and 2.33 nM. Third, the assay forms in undiluted human serum (though the electrochemical analysis is carried out in buffer). Second, the assay is selective in that it does not cross-react with other cardiac natriuretic peptides. First, compared to physisorption of the detection Abs on the AgNP labels, a 27-fold increase in signal is observed when a heterobifunctional cross-linker is used to facilitate this labeling. There are four important outcomes reported in this article. Sensing of NT-proBNP relies on the formation of a sandwich immunoassay and electrochemical quantification of silver nanoparticle (AgNP) labels attached to the detection antibodies (Abs). The approach is based on a paper electrode assembly and a metalloimmunoassay it is intended for eventual integration into a home-use sensor. Dealing with violence and aggression.In this paper, we demonstrate an electrochemical method for detection of the heart failure biomarker, N-terminal prohormone brain natriuretic peptide (NT-proBNP). Breaking my leg in October 1994 in my second kickboxing fight, which gave me an opportunity to develop my Coaching ability. Supporting John Scida in the corner over the years.Ĭurrent Interests: Teaching & researching martial arts Fluid Adaption Martial Arts as psychotherapy MMA collecting Martial Arts book and reading them all. Fighting as a Heavyweight Kick-boxer in 1994. Producing my first Zen Do Kai Black Belt Peter Pum at my Bacchus MarshDojoin 1993. Working Security for 15 years.Ĭareer Highlights: Receiving my Black Belt and Gold Bushido Cross from John Scida. Main I nfluences: John Scida, Frank Lanciana, Peter Alexsopoulis Bob Jones, Mitch Bayliss, Sam Zarafa, Machine Gun Charlie, Frank DiBlasi, Rick Bradley, Joe Tosic, Steve Aldersly, John Beckman and Ray Cannon. Key strengths: Coaching, Pad Holding for Boxing, Kickboxing and Muay Thai, Mixed Martial Arts specific striking, Sparring in Boxing, Kickboxing and MMA. Instructor at: Melbourne - Western Bulldogs
#John scida free#
Many times winner in the Wildcats association fight team, numerous free fight tournament wins, performing advanced martial arts demonstrations at the Melbourne Town hall and many shopping centres, planning and managing the Wildcat annual Senjo grading’s with up to 100 participants each year, being a member on the Wildcat steering committee, (setting policy and direction).Ĭurrent Interests: Continuous learning in the martial arts, helping people be the best they can be in mind, body and spirt, through my own life’s learnings and my martial arts ability.įoundation Arts: Judo, Zen Do Kai, Kickboxing Head instructor at the Broadmeadows Dojo, producing 80+ black belts, twice Victorian Champion in Weapon combat and Weapon Kata. Main I nfluences: Brian Agar, Raffaele Lanciana, Maurice Garnier, Bob Jones, Rod Catterall, Percy Lanciana, Joe Lanciana, Mitch Bayliss, and Paul Basford.Ĭareer Highlights: Being awarded my first Black belt at the Melbourne Town hall Senjo in 1983. Key strengths: teaching, kicking ability and weapons, (Katana / Sai / Walking stick) Started Martial Arts: September 1979, aged 16 Watching Bruce Lee movies, reading martial arts classics, chaos theory, psychologyĬareer Highlights: Bulleen Bobcat Instructor 1983 - 1998 BJC Best Up and Coming Tournament Fighter 1982 ZDK Free Fighting champion 1985 (Novice Black) and 1991 (Advanced Black) ZDK Free Combat Champion 1985 tournament battles against Daryl Davis watching Percy Lanciana and Andrew Ramacciotti complete Nidan Self Defence classes with Soke Bob Jones Black Belt instructor on BJC Wildcat Summer Camps.Ĭurrent Interests: Teaching & researching martial arts Reality Based Martial Arts Meditation Martial Arts as psychotherapy Okinawan Goju Ryu Karate exploring the relationship between patterns (Kata), combat and life keeping fit, healthy, and generally ageing well. Main Influences: Percy Lanciana, Raffaele Lanciana, Maurice Garnier, Bob Jones, Suzanna Daily, Paul Fyfeild, Mark Beverstock, and Enis Murtic.
Key Strengths: teaching, free fighting, hand to hand defence, street and traditional weapons, meditation, creativity and innovation in the martial arts.
Started Martial Arts: 7 March 1979, aged 14
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